Introduction: Around the world, 6% of infants are said to be born with a congenital abnormality, which causes countless fatalities. However, since statistics sometimes do not take aborted pregnancies and stillbirths into account, the actual number of cases may be significantly greater. With 753,000 neonatal fatalities reported in 2013, the nation's neonatal mortality rate was 29 per 1000 live births. As there are limited data available on fetal congenital anomalies it is important to educate mothers and women. Objectives: This study was conducted to determine the knowledge of the prevention of fetal congenital anomalies. Methodology: A pre-experimental study with one group pre-test and post-test design was undertaken to conduct this study from 20 august to 30 October using a convenience sampling technique. A total of 60 mothersparticipated in the study through a structured knowledge questionnaire. Data was collected through pen and paper and exported to SPSS version 20.0 for further analysis.The descriptive statistics were presented with mean ± standard deviation, frequency, and percentage. The data were analyzed using a paired t-test. The chi-square analysis was used to check the association. Results: Results of the study revealed that at the pre-test level 47(78.3%) of the study participants had inadequate knowledge, 13(21.7%) of them had moderate knowledge, and none of the study participants were having adequate knowledge. Whereas after the intervention majority 43(71.7%) had moderateknowledge, 17(28.3%) of them had adequate knowledge, and none of the study participants had inadequate knowledge regarding congenital anomalies. The paired “t” test was carried out and it was found significant at p<0.05 level inage and knowledge level (χ2=6.60, df=2). Furthermore, the association between type of family (χ2=12.11, df=2), family history of congenital anomalies (χ2=17.17, df=1), and source of health information(χ2=8.52, df=3) werealso found to be statistically significant. Conclusion: Our data suggest that the mothers who are residing in the rural districtsof Bengaluru have significantly less knowledge regarding prevention of congenital anomalies. so, it is very important to make sure that women and mothers should know about congenital anomalies and how to manage and prevent it. Our findings will support the stakeholders, nurses, community health workers, public health professionals, and government policymakers to make a significant decision to improve public knowledge related to congenital anomalies.