In this study, to confirm the antiobesity efficacy of wheat germ hot-water extract, hot-water extraction with 20%, 50%, and 80% ethanol content was used to analyze antioxidant efficacy, Staphylococcus aureus antibacterial activity, lipase inhibitory activity, and α-glucosidase activity inhibitory activity. As a result of the antioxidant test of wheat germ extracts, the IC50 values of ascorbic acid used as a control was 2.16, whereas the values for 20%, 50%, and 80% ethanol of wheat germ extracts were 3.13, 2.91, and 2.64, respectively. The values were lowers in all samples than that in ascorbic acid, but antioxidants showed concentration-dependent effects. The result of the antibacterial analysis of wheat germ extracts showed that the antimicrobial activity increased as the ethanol content increased, and specifically, the 80% ethanol extract showed a high bacterial reduction rate of 75.7%. In the lipase activity inhibition analysis of wheat germ extracts, the lipase inhibitory activity of orlistat (positive control) was 81.9% ± 1.17%, whereas those of 20%, 50%, and 80% ethanol extract were 76.0% ± 7.42%, 85.5% ± 5.01%, and 95.5% ± 3.26%, respectively. All extracts showed lipase inhibitory activity. Specifically, 80% ethanol extract showed higher lipase inhibitory activity than orlistat (positive control). In the α-glucosidase activity inhibitory activity analysis of wheat germ extracts, the inhibitory activity of acarbose (positive control) was 87.5% ± 3.21%; 20% ethanol extract, 71.3% ± 3.40%; 50% ethanol extract, 88.2% ± 4.00%; and 80% ethanol extract, 90.2% ± 90.2 ± 4.00%. All extracts showed α-glucosidase inhibitory activity at 2.12%. Specifically, the 80% ethanol extract showed a higher α-glucosidase inhibitory activity than acarbose (positive control). This is caused by the effect of complex extracts such as 9,12-octadecadienoic acid, linoleic acid ethyl ester, and linoelaidic acid.
