Hospital infections are considered as a cause of the high mortality rate that occurs during the hospitalization period, and considered an important public health problem. Pseudomonas aeruginosa, Klebsiella pneumoniae and Staphylococcus aureus are among the most frequent, easily transmitted and resilient microorganisms resistant to several antimicrobials. The aim of this research was to investigate the prevalence of P. aeruginosa and K. pneumoniae among undergraduate students of Health Sciences area who do not attend the hospital environmentand test the sensitivity of these pathogens to antimicrobial agents. A total of 120 nasal material samples were analyzed. Isolation and identification of bacteria were performed by microbiological methods. The results showed that among the 120 examined students, 3 were colonized by P. aeruginosa, with a prevalence of 2.5%, and 2 colonized with K. pneumoniae with prevalence of 1.7%. No multidrug resistant strains were isolated. All strains showed variable degrees of susceptibility to the tested antimicrobials.