Simultaneous estimation of levodopa and benserazide by using reverse phase high Performance liquid chromatography in api and marketed formulation
Pharmaceutical analysis plays a vital role in the Quality Assurance and Quality control of bulk drugs. A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of benserazide and levodopa, in its pure form as well as in tablet dosage form. Chromatography was carried out on an Altima C18 (4.6 x 150mm, 5µm) column using a mixture of methanol: TEA buffer pH 4.5: acetonitrile (50:25:25) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 225nm.