The study aims to compare unilateral squat jump performance between the non-dominant and dominant leg in male handball players. To achieve the purpose 38 male handball players were selected as subjects from Annamalai University. Unilateral squat jump data was obtained using Gyko Inertial system (Microgate, s.r.l., Italy) and optojump (Microgate, s.r.l., Italy).Paired t test was administered to ascertain the unilateral difference in squat jump performance between non-dominant and dominant leg. The paired sample t test showed significant difference between non-dominant leg and dominant leg in flight time, maximum velocity and maximum power. Remaining Variables failed to show significant difference (p> 0.05). It is concluded that male handball players exhibit significantly greater flight time, maximum velocity, and maximum power in the dominant leg compared to the non-dominant leg during unilateral squat jump performance.