Cygb is an O2 carrier protein expressed by fibroblasts. In fibrosis, hypoxia occurs as characterized by stabilization of hypoxia-inducible factor-1α (HIF-1α), which later form the HIF-1, a transcription factor for the expression of adaptation proteins (including Cygb). The purpose of this study was to obtain information about Cygb role in fibrosis hypoxia using keloid tissue as a model. This was an observational descriptive study using keloid tissue samples, and the preputium as control. Measurement of Cygb and HIF-1α mRNA expression by real-time RT–PCR. Cygb and HIF-1α protein level (ELISA); while Cygb and HIF-1α protein expressions in the dermis layer by IHC. Data were analyzed statistically using unpaired t-test. In keloid, Cygb mRNA expression increased 8.7 times, compared to preputium, Cygb protein increased significantly (1.19 Vs 0.78 ng/mg protein and 95% Vs 63%, P<0.05). HIF-1α mRNA expression increased by 5.1 times, in keloid tissue, and protein HIF-1α increased significantly (0.20 Vs 0.12 ng/mg protein and 80% Vs 38%, P<0.05). There is a strong positive correlation between the expression of the HIF-1α and Cygb mRNA (Pearson; R = 0.899, P = 0.000).The expression of cytoglobin (Cygb) increased in hypoxia fibrosis tissue with keloid.
