Lipase production from myroides odoratimimus (sks 05) and hptlc analysis of purified lipase

Background: Microbes play an important role in the area of biodegradation. In order to reduce the damages caused due to oil spills, preliminary study on the role of Myroides odoratimimus obtained from chicken in the biodegradation of diesel hydrocarbons. Objectives: Production of Lipase from Myroides odoratimimus (SKS 05) through solid state fermentation using different substrates and characterization of Lipase enzyme produced from fermentation. Checking its purity through HPTLC analysis. Methods: Lipase enzyme production through submerged and solid state Fermentation. Selection of substrate to increase lipase production and check the effect of various parameters in lipase activity like pH, temperature, incubation period, carbon source and moisture level. Partial purification of lipase using column chromatography and quantification of partially purified lipase using HPTL. Results: The present study reports that the production of lipase by Myroides odoratimimus (SKS 05) using different oil cakes. Groundnut oil cake was found suitable for maximum lipase production and its activity increases at temperature 35 to 40 °C, pH8, moisture level from 50 to 60% and glucose used as a sole carbon source. Conclusions: Lipase production from Myroides odoratimus can be enhanced using substrates and maintaining optimium conditions. The produced lipase can be used for industrial as well as biodegradation purpose