Detection of genetic polymorphisms among commercial tea population and endemic wild tea plant (gordonia obtusa. Exut and arn) as revealed by rapd markers

RESEARCH ARTICLE: 
Shalimol, A., Arumugasamy, K., Nantha kumar, R., Abdul kaffoor, H., Muthuraj, K., Midhusha Johny and Minu Venugopal

Genomic fingerprinting of 4 tea plant genotypes was carried out using 7 RAPD multiloci primers. All the primers showed significant amplification in PCR Analysis. A total of 303 bands were produced in all the 4 clones with an average of 43.29% RAPD bands per primer. Among all the bands 16.83% were polymorphic in nature. The molecular size of the amplified DNA fragments ranged from 100 to 6000 bp. Dendrogram was constructed based on the genetic similarity matrix using the UPGMA algorithm, which showed two main clusters. Cluster 1 contains only one clone i.e., U3 and the reminder 3 clones in cluster 2. Cluster 2 was further sub-divided into 2 sub-clusters. In which wild type tea plant categorized in 1 subcluster and the remaining two tea clones are categorized to form sub-sub clusters. Cluster analysis revealed that all the four genotypes were widely diverse genetically. All the four genotypes were showed identical polymorphism while generating phylogenetic tree.

Paper No: 
277
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