Hydrocotyle conferta Wight is an endangered plant species in Southern Western Ghats. Methanolic whole plant extract of H. conferta study was investigated for various DPPH, ABTS, hydroxyl radical scavenging, reducing power and ferrous ion chelating assays. The results of DPPH activity showed that the extract at the dose of 50μg/ml has exhibited in 89.15±0.16 inhibition with an IC50 value of 34.21±0.52mg/ml. The highest ABTS scavenging activity showed 762.63±0.53 trolox equivalence in μMol/g extract at the dose of 50μg/ml extract, Hydroxyl radical scavenging effect of the methanolic extract at the concentration of 50μg/ml was found to be 69.16±0.31. The reducing power assay showed the 0.79 absorption at 720 nm extract at the dose of 50μg/ml, and metal chelating activity at concentrations of 50μg/ml extract showed 67.52 ±0.03 suggested. It is that antioxidant activity of crude methanolic extract could be used as a source of natural antioxidants of H. conferta and needs further stud to bring new natural products into pharmaceutical industries.
