Neisseria gonorrhoeae, chlamydia trachomatis, ureaplasma urealyticum, mycoplasma hominis and mycoplasma genitalium
The identification of sexually transmitted pathogens by rapid and sensitive techniques is of interest due to the spread routes including the risks of acquisition and transmission. Our aim was to develop multiplex real-time PCR assays to simultaneously detect M. hominis, M. genitalium, U. urealyticum, N. gonorrhoeae, and C. trachomatis. Clinical samples from female patients with presumptive diagnosis of infection with these bacteria were examined. Two multiplex real-time PCRs were developed: MI: N. gonorrhoeae, C. trachomatis, U. urealyticum; MII: M. hominis, M. genitalium, U. urealyticum.